x. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. The. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. The system can perform readings. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. 0. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. Fluorescence Microplate Reader Comparison Chart. Temperature variation ± 0. They can be read by most microplate readers. We would like to show you a description here but the site won’t allow us. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Values are averages from 5 clean plates, each filled with 200 µL water/well. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. x. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 1% in white 96- and < 0. 96-, 384-, and 1536-well microplates. These robust readers have been placed in labs from Antarctica to the. Figure 1-2 Match chamfered corners of slide and adapter 2. HeLa. • 0. 100 fmol luciferase per well. Turn the instrument around so that the back of the instrument is. A = Log 10 (I 0 /I). Bowman Dispensers. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. PURPOSE. ₹ 1,80,000 Get Latest Price. 96-, 384-, and 1536-well microplates. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. 306 plate air cal. These plates are compatible with standard fluorescence microplate readers and are offered in a. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. 000 OD ± 1. Compare this item. flat-bottom: 50-60 µl. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Features. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Click on the crosshair and move it so that it is centered over the well image. M2. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. * Note: Programming the washer with a final aspirate height of 4. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Range of 96, 384-well SBS microplates . None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. From the dropdown Menu, select Wellplates. 190. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. The advanced design of the Sunrise plate reader includes. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. x. 96-, 384-, and 1536-well microplates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 0 OD. White 96-well plate Plate formats. . 96 well plate injection time with SpectraMax® L plate reader. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. #ABS PLUS) Methods. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. Selects either 96-well or 384-well plate for a stand-alone read. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. EMax. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 000 – 4. x. Pick the best speed, sensitivity, and price for your needs. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. x. Now select the area of the plate to read. measurements in compatible Molecular Devices® microplate readers. VMax . 384-WELL SBS STERILE MICROPLATE. Learn how you can utilize microplate readers to drive landmark discoveries. Turn on Plate Reader and wait for it to complete its start-up routine. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. An industry-renowned instrument for monitoring GPCRs and ion channels. Working in. Uncontrolled Copy for Training and Reference Purposes Only . Molecular Spectroscopy Consumables & Accessories Thermal Analysis. with Quartz ABS Plastic. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. Instrument settings can. 5L) MultiWash+. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 00. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. 25, 3. The VICTOR Nivo. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. VIS absorbance detection. EMax. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. 4 decades . 100 μL of working reagent was added to each well after the 10-minute preincubation step. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. These lab workhorses are used in drug discovery. 5 second read per plate. The IC50s by cell rounding and LysoTracker. We would like to show you a description here but the site won’t allow us. NanoQuant plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 00 . Gemini EM Reader scans. External Dimensions without lid (mm). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. Plate chamber design of the SpectraMax category 1 microplate readers. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. minutes and begin reading microplates with seven mouse clicks. x. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Supersedes 1. % transmittance of plates used in a plate reader. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. The CellTiter-Glo assay was used to measure ATP. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. 130009WMicroplate Readers. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. ©2022 Molecular Devices - All rights. Packed 25 plates per sleeve, 2 sleeves per case. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Microplate Reader. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. #SpectraMax L Config) Methods. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. a white 96-well half-area microplate (Corning cat. High tolerance for contaminants. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Compare specifications and request a quote from leading suppliers. Corning. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. This page contains the. 100 fmol 1536-well (8 µL) 0. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. 984. Lucid Scientific. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. x. Tristar 3 Multimode Microplate Reader. #C1500) Control standard endotoxin (ACC cat. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. No. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. $488. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. d. Figure 2. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. • High antibody-binding surface improves the overall performance in. Sort By: Show:. robotics-automation-compatible. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. When the user applies a set temperature of 37°C, the top is heated to 37. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Endpoint, kinetic, spectrum. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. 2. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. QC tools and IQOQ services. Versa Max. 9. x. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Populate the following info under Description. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. 1. Corning. and the A1 corner of the plate adapter (Figure 1-2). FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. Products : General, FilterMax F5 reader. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. 1 mL/well of media. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). These microplates have a corner notch for use in standard plate readers. to each well. absorbance microplate reader GEA. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Mode Plate Reader. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 76358-624. 000 OD ± 1. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. PURPOSE. VMax . Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. adjust the reader. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. Plate Background OD, which was used for subsequent tests, was measured as follows. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. (one set per assay) to clear 96-well microplates. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Quantitative, whole well cell confluence for every well. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. Materials. 96-well-plate-scan-time-2-colors-1-site-well. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. It features a modular design that allows users to add or change detection cartridges according to their needs. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 0025 OD from 0. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Click Create. Use standard plate unless you see your type listed. Intuitive graphical user interface makes each assay extremely easy to set. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. p-Nitroaniline - Catalog No. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Fluorescence Microplate Reader Comparison Chart. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. 1. 1 OD at 230 nm. 4 (Molecular Devices cat. Product Compare 0. This can increase throughput and efficiency, thus saving time and space in the lab. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Or scan up to 384 wells at any time to determine the best assay conditions. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. Microplates, cuvettes (via adapter) Detectors. Published Dec 16,. HeLa. $14,995. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. g. Category 2 and 3 microplate readers use gradient heating as well. Plate. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. White 96-well platePlate formats. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. . Part Number. 315 can't find zero order. We would like to show you a description here but the site won’t allow us. • Photometric range: 0. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Figure 1. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. Integrated stacker option. Reconstitution of. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Molecular Devices has provided scientists with . By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Step 3: Click “Settings” and a screen will appear (Figure 1). It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. The opaque walls prevent stray light from being detected from neighbouring. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. x. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Selects either 96-well or 384-well plate for a stand-alone read. 96- and 384-well. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 340 PC384. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Dual injectors for kinetic assays. Other. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. 0 . These robust readers have been placed in labs from. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Published Dec 16, 2020 | Updated Dec 16, 2020. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Get Quote . The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. 2% in. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. With various on-board features and capabilities, the ThermoMax. Running both standard spectrophotometer and microplate. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. #N207) LAL Reagent Water (Lonza cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2. x. 00 Add to cart; Molecular Devices EMax Microplate Reader $. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Selects either 96-well or 384-well plate for a stand-alone read. Enter the name of the plate type i. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Controlled by the computer. Figure 2-23: Bottle holder To install the bottle holder: 1. Sample by eye within . The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. No. Component A (2 vials*)Figure 1. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. minutes and begin reading microplates with seven mouse clicks. Wavelength(s):. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 1 fmol luciferase per well. 5°C at 37°C. It features a modular design that allows users to add or change detection cartridges according to their needs.